Synthesis, characterization and evaluation of antibacterial properties of Aloe vera / chondroitin sulfate / nanocellulose nanocomposite hydrogel. / M. R. Mohammad Shafiee, P. Farsaiee Nejad, Z. Shams Ghahfarokhi. / Nano Studies. – 2021–2022. – # 21/22. – pp. 63-74. – eng. The synthesis of nanocomposite hydrogel as an antibacterial agent is reported. The nanocomposite hydrogel was synthesized using freeze-drying method by blending Aloe vera, chondroitin sulfate and nanocellulose. The synthesized nanocomposite was characterized using Fourier transform infrared (FT–IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The evidence showed that there is a noteworthy chemical interaction between the Aloe vera, chondroitin sulfate and nanocellulose. In addition, equilibrium degree of swelling of nanocomposite hydrogel was determined gravimetrically. The antibacterial studies of Aloe vera / chondroitin sulfate / nanocellulose nanocomposite hydrogel was evaluated against Escherichia coli (gram-negative) and Staphylococcus aureus (gram-positive) bacteria. The results illustrated that the nanocomposite hydrogel has good antibacterial activity against gram-positive and gram-negative bacteria and can be suitable for antimicrobial applications. Fig. 7, Ref. 39.